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      Xiburema Virus, a Hitherto Undescribed Virus within the Family Rhabdoviridae Isolated in the Brazilian Amazon Region

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          Abstract

          We report here the first complete open reading frame (ORF) genome sequence of Xiburema virus (XIBV), that of strain BE AR362159, isolated from mosquitoes ( Sabethes intermedius) in Sena Madureira, Acre state, northern Brazil. All genes showed similarities with those belonging to members of the family Rhabdoviridae.

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          An integrated semiconductor device enabling non-optical genome sequencing.

          The seminal importance of DNA sequencing to the life sciences, biotechnology and medicine has driven the search for more scalable and lower-cost solutions. Here we describe a DNA sequencing technology in which scalable, low-cost semiconductor manufacturing techniques are used to make an integrated circuit able to directly perform non-optical DNA sequencing of genomes. Sequence data are obtained by directly sensing the ions produced by template-directed DNA polymerase synthesis using all-natural nucleotides on this massively parallel semiconductor-sensing device or ion chip. The ion chip contains ion-sensitive, field-effect transistor-based sensors in perfect register with 1.2 million wells, which provide confinement and allow parallel, simultaneous detection of independent sequencing reactions. Use of the most widely used technology for constructing integrated circuits, the complementary metal-oxide semiconductor (CMOS) process, allows for low-cost, large-scale production and scaling of the device to higher densities and larger array sizes. We show the performance of the system by sequencing three bacterial genomes, its robustness and scalability by producing ion chips with up to 10 times as many sensors and sequencing a human genome.
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            A Large-Scale Outbreak of Bovine Ephemeral Fever in Turkey, 2012

            ABSTRACT Regional cases of bovine ephemeral fever (BEF) were documented previously in Turkey. Previous cases were confirmed in South-East Turkey with low cow mortality. Recent BEF-suspected outbreaks with high mortality were documented in many regions of Turkey in 2012. The aim of study was the epidemiological examination of the outbreak and molecular characterization of the viruses detected from the outbreak. For this reason, blood samples were collected from BEF-suspected outbreak regions. From the results of RT-PCR, high rate of BEF-suspected samples (48/60 or 80%) was found positive for BEF virus (BEFV) RNA. The nucleotide sequences of the G1 region of G gene of BEFV in the current study during the 2012 outbreak were grouped into cluster II of BEFV. It was suggested that BEFV may be spread out to other neighbor countries in the future years.
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              International Catalogue of Arboviruses, Including Certain Other Viruses of Vertebrates

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                Author and article information

                Journal
                Genome Announc
                Genome Announc
                ga
                ga
                GA
                Genome Announcements
                American Society for Microbiology (1752 N St., N.W., Washington, DC )
                2169-8287
                19 June 2014
                May-Jun 2014
                : 2
                : 3
                : e00454-14
                Affiliations
                [a ]Department of Virology, Evandro Chagas Institute, Ministry of Health, Ananindeua, Brazil
                [b ]Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, Brazil
                [c ]Department of Electron Microscopy, Evandro Chagas Institute, Ministry of Health, Belém, Brazil
                [d ]Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Brazil
                Author notes
                Address correspondence to Ana Lucia M. Wanzeller, anawanzeller@ 123456iec.pa.gov.br , or Márcio R. T. Nunes, marcionunes@ 123456iec.pa.gov.br .
                Article
                genomeA00454-14
                10.1128/genomeA.00454-14
                4064021
                ce2d2c35-2ea6-4535-8aad-84c3d18aed4f
                Copyright © 2014 Wanzeller et al.

                This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

                History
                : 24 April 2014
                : 6 June 2014
                Page count
                Pages: 2
                Categories
                Viruses
                Custom metadata
                May/June 2014
                free

                Genetics
                Genetics

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