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      A Spontaneous Deletion of the US1.67/US2 Genomic Region on the Bovine Herpesvirus 1 Strain Cooper

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          Abstract

          Bovine herpesvirus 1 (BoHV-1) is an alphaherpesvirus with a genome of 135 kb. Some BoHV-1 genes are nonessential and may be deleted from the viral genome. Here, a spontaneous gene deletion was identified in the BoHV-1 strain Cooper. Genes of the US1.67/US2 region were absent, as determined by next-generation sequencing.

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          Genomic and Functional Characteristics of Human Cytomegalovirus Revealed by Next-Generation Sequencing

          Steven Sijmons, Marc Van Ranst, Piet Maes (2014)
          The complete genome of human cytomegalovirus (HCMV) was elucidated almost 25 years ago using a traditional cloning and Sanger sequencing approach. Analysis of the genetic content of additional laboratory and clinical isolates has lead to a better, albeit still incomplete, definition of the coding potential and diversity of wild-type HCMV strains. The introduction of a new generation of massively parallel sequencing technologies, collectively called next-generation sequencing, has profoundly increased the throughput and resolution of the genomics field. These increased possibilities are already leading to a better understanding of the circulating diversity of HCMV clinical isolates. The higher resolution of next-generation sequencing provides new opportunities in the study of intrahost viral population structures. Furthermore, deep sequencing enables novel diagnostic applications for sensitive drug resistance mutation detection. RNA-seq applications have changed the picture of the HCMV transcriptome, which resulted in proof of a vast amount of splicing events and alternative transcripts. This review discusses the application of next-generation sequencing technologies, which has provided a clearer picture of the intricate nature of the HCMV genome. The continuing development and application of novel sequencing technologies will further augment our understanding of this ubiquitous, but elusive, herpesvirus.
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            Spontaneous BHV1 recombinants in which the gI/gE/US9 region is replaced by a duplication/inversion of the US1.5/US2 region.

            J Madic, S B Verschuren, F Rijsewijk … (1999)
            In a bovine herpesvirus 1 (BHV1) vaccine strain, a spontaneous BHV1 mutant (Za) was found that arose from a recombination between two isomeric forms of the BHV1 genome. In this Za mutant one end of the U(S) region, containing part of the US1.5 gene, was found duplicated in an inverted orientation at the other end of the U(S) region. Concurrently, a 2.7 kb deletion was found in Za that encompasses both the US8 (gE) and US9 gene. Analysis of the in vitro growth properties of a genetically modified BHV1gE(-) mutant showed that at 11 hours post infection BHV1gE(-) viruses were secreted ten times more efficiently than wild type virus. Using this observation we developed a protocol to enrich for spontaneous gE deletion mutants in a BHV1 field isolate and found another mutant (Rof3) with similar properties as the Za mutant. Rof3 has a duplication/inversion of the US1.5 gene and part of the US2 gene and a simultaneous 3.5 kb deletion that encompasses the US7 (gI), US8 (gE) and US9 genes. The nucleotide sequences of the recombination points of both recombinants were determined and compared. No obvious sequence similarities were found, suggesting that non-homologous recombination events led to the observed recombinations. The implications for the use of BHV1 gE deletion mutants as marker or diva vaccines are discussed.
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              Immunogenicity of 2 infectious bovine rhinotracheitis vaccines.

              F ZUSCHEK, T. Chow (1961)
              Article 0 comments Cited 1 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Genome Announc
                Journal ID (iso-abbrev): Genome Announc
                Journal ID (hwp): ga
                Journal ID (pmc): ga
                Journal ID (publisher-id): GA
                Title: Genome Announcements
                Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )
                ISSN (Electronic): 2169-8287
                Publication date (Electronic): 4 February 2016
                Publication date Collection: Jan-Feb 2016
                Volume: 4
                Issue: 1
                Electronic Location Identifier: e01665-15
                Affiliations
                Laboratório de Virologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
                Author notes
                Address correspondence to P. M. Roehe, proehe@ 123456gmail.com .
                Article
                Publisher ID: genomeA01665-15
                DOI: 10.1128/genomeA.01665-15
                PMC ID: 4742685
                PubMed ID: 26847888
                SO-VID: d2626ccd-49ba-4cb6-a511-6ab859d8a97f
                Copyright © Copyright © 2016 Campos et al.
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                Date received : 2 December 2015
                Date accepted : 18 December 2015
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 5, Pages: 1, Words: 794
                Funding
                Funded by: MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) http://dx.doi.org/10.13039/501100003593
                Award Recipient : Ana Claudia Franco
                Funded by: Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) http://dx.doi.org/10.13039/501100004263
                Award Recipient : Fabrício Souza Campos
                Funded by: MCTI | Financiadora de Estudos e Projetos (FINEP) http://dx.doi.org/10.13039/501100004809
                Award Recipient : Paulo Michel Roehe
                Categories
                Subject: Viruses
                Custom metadata
                cover-date January/February 2016

                ScienceOpen disciplines: Genetics
                Data availability:
                ScienceOpen disciplines: Genetics

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