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      Split-spectrum amplitude-decorrelation angiography with optical coherence tomography


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          Amplitude decorrelation measurement is sensitive to transverse flow and immune to phase noise in comparison to Doppler and other phase-based approaches. However, the high axial resolution of OCT makes it very sensitive to the pulsatile bulk motion noise in the axial direction. To overcome this limitation, we developed split-spectrum amplitude-decorrelation angiography (SSADA) to improve the signal-to-noise ratio (SNR) of flow detection. The full OCT spectrum was split into several narrower bands. Inter-B-scan decorrelation was computed using the spectral bands separately and then averaged. The SSADA algorithm was tested on in vivo images of the human macula and optic nerve head. It significantly improved both SNR for flow detection and connectivity of microvascular network when compared to other amplitude-decorrelation algorithms.

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          Most cited references42

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          Optical coherence tomography.

          A technique called optical coherence tomography (OCT) has been developed for noninvasive cross-sectional imaging in biological systems. OCT uses low-coherence interferometry to produce a two-dimensional image of optical scattering from internal tissue microstructures in a way that is analogous to ultrasonic pulse-echo imaging. OCT has longitudinal and lateral spatial resolutions of a few micrometers and can detect reflected signals as small as approximately 10(-10) of the incident optical power. Tomographic imaging is demonstrated in vitro in the peripapillary area of the retina and in the coronary artery, two clinically relevant examples that are representative of transparent and turbid media, respectively.
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            Age-related macular degeneration is the leading cause of blindness...

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              Speckle variance detection of microvasculature using swept-source optical coherence tomography.

              We report on imaging of microcirculation by calculating the speckle variance of optical coherence tomography (OCT) structural images acquired using a Fourier domain mode-locked swept-wavelength laser. The algorithm calculates interframe speckle variance in two-dimensional and three-dimensional OCT data sets and shows little dependence to the Doppler angle ranging from 75 degrees to 90 degrees . We demonstrate in vivo detection of blood flow in vessels as small as 25 microm in diameter in a dorsal skinfold window chamber model with direct comparison with intravital fluorescence confocal microscopy. This technique can visualize vessel-size-dependent vascular shutdown and transient vascular occlusion during Visudyne photodynamic therapy and may provide opportunities for studying therapeutic effects of antivascular treatments without on exogenous contrast agent.

                Author and article information

                Opt Express
                Opt Express
                Optics Express
                Optical Society of America
                09 February 2012
                13 February 2012
                09 February 2013
                : 20
                : 4
                : 4710-4725
                [1 ]Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA
                [2 ]Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089, USA
                [3 ]Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
                [4 ]Advanced Imaging Group, Thorlabs, Inc., Newton, NJ 07860, USA
                [5 ]Pattern Recognition Lab, University Erlangen-Nuremberg, D-91058 Erlangen, Germany
                Author notes
                ©2012 Optical Society of America

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

                Funded by: NIH
                Award ID: R01 EY013516
                Award ID: R01-EY11289-26
                Funded by: AFOSR
                Award ID: FA9550-10-1-0551
                Custom metadata

                Ophthalmology & Optometry
                (170.4500) optical coherence tomography,(170.3880) medical and biological imaging,(170.4470) ophthalmology,(999.999) optical angiography


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